Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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Facts-enriched characterization of mobile populations. Morphological info from photographs can increase into the richness of flow cytometry information. Such as, the determine shows an or else standard apoptosis assay using Annexin V and PI, incorporating mobile imaging to characterize cells in Every population to expose morphologically distinctive characteristics. These insights could not happen to be acquired from multiplex staining on your own.
As samples are obtained within the Attune CytPix Flow Cytometer, the high-velocity brightfield digital camera captures and outlets visuals of detected functions in a price as much as six,000 images for every second, depending on the flow amount and graphic measurement. For increased versatility, Attune Cytometric Software program enables you to alter the impression seize frequency as required and choose a selected gate for picture capture, if necessary.
(B) Uncooked 264.7 macrophages are often annotated inaccurately with respect to particle depend due to their complexity and noncircular borders. The amount of singlets available for analysis boosts when the user trains and employs a more precise model.
Uncover new insights into your experimental data by visualizing populations employing put together fluorescence and morphology information. Our software program interface wizards help downsampling, concatenation (merged information) and dimensionality applications which have been crafted in to the software program for an intuitive, streamlined details analysis encounter.
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In addition, it can be illuminating to view how many doublets slip by way of conventional singlet gates, and terrific to validate the status of unforeseen double-positive gatherings in my Examination."
Pair your Attune flow cytometer using an autosampler for amplified workflow efficiency, and incorporate robotic integration for optimum throughput.
Our scientists will layout panels to suit your needs as again and again you require—cost-free, no product buy wanted.
The Attune NxT cytometer could be the trustworthy workhorse instrument your lab can depend on for consistent and reliable effectiveness. The Attune CytPix model includes a significant-speed brightfield digicam and AI-pushed impression Examination software package, with current updates together with machine Finding out applications and an intuitive person interface, enabling Increased facts precision, accuracy, and a lot quicker insights into sample biology.
Potentially most of all, these activities contain cells of Obviously different phenotypes which could lead to incorrect conclusions about double good gatherings (specifically in uncommon populations).
Sound waves empower precise mobile alignment, resulting in Fantastic knowledge high-quality and ~10X more quickly sample flow rates.
We Beforehand demonstrated the power of imaging CAR-T/Ramos cell interactions. Allow’s evaluate just the population of finest interest, the double beneficial gatherings, to learn more. We could now use extended morphological parameters (right here, circularity and skewness of intensity) to additional analyze the attributes of these populations and refine gating on these functions, rising details robustness.
Leverage the strength of Sophisticated engineering and innovation to speed up your discoveries. Believe in during the Attune Flow Cytometer family members to deliver the trustworthiness, pace, and accuracy that established your perform apart.
Adaptable payment program from Agilent Money Answers enable you to obtain the important instrumentation you'll need for rapid project, or to expand your lab.
Gating on these prolonged parameters allows you to swiftly and properly determine populations of desire and confirm gating tactic with little or no manual evaluation. The Attune software program offers access to point out-of-the-art device Understanding Investigation resources inside an expertly designed person interface, which makes it extremely convenient to use.
Since the cell line had Formerly been analyzed on the Attune CytPix Flow Cytometer, the scientists went again to the pictures and ended up ready to document the microbial an infection a minimum of five days previously.
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Below, we show that by gating approaches working with picture-based mostly quantitative parameters can distinguish interacting cells from coincident activities to a lot more correctly assess interacting cells.